52332
Phenylmethylsulfonyl Fluoride
Phenylmethylsulfonyl Fluoride, CAS 329-98-6, is an irreversible inhibitor of serine proteases. It causes sulfonylation of the active-site serine residues.
Synonym(s):
Phenylmethylsulfonyl Fluoride, Benzylsulfonyl Fluoride, PMSF
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Empirical Formula (Hill Notation):
C7H7FO2S
CAS Number:
Molecular Weight:
174.19
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.77
Recommended Products
Quality Level
Assay
≥99% (GC)
form
crystalline solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated
color
white to off-white
solubility
ethanol: soluble
isopropanol: soluble
methanol: soluble
shipped in
ambient
storage temp.
10-30°C
InChI
1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2
InChI key
YBYRMVIVWMBXKQ-UHFFFAOYSA-N
General description
Irreversible inhibitor of serine proteases. Its mechanism of action is analogous to that of diisopropylfluorophosphate. PMSF causes sulfonylation of the active-site serine residues. Also reported to inhibit internucleosomal DNA fragmentation in immature thymocytes. Effective at a concentration of 50 µM.
Irreversible inhibitor of serine proteases. Its mechanism of action is analogous to that of diisopropylfluorophosphate. PMSF causes sulfonylation of the active-site serine residues. Also reported to inhibit internucleosomal DNA fragmentation in immature thymocytes. For a related, more stable inhibitor, see AEBSF (Cat. No. 101500).
Biochem/physiol Actions
Cell permeable: no
Primary Target
serine proteases
serine proteases
Product does not compete with ATP.
Reversible: no
Warning
Toxicity: Corrosive (L)
Reconstitution
Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 9 months at 4°C.
t½ = 1 hour at pH 7.5. Effective concentration: 50 μM.
Other Notes
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Seitz, R., et al. 1993. Int. J. Cancer53, 514.
Weaver, V.M., et al. 1993. Biochem. Cell. Biol.71, 488.
Bourgain, R.H., et al. 1992. Adv. Exp. Med. Biol.316, 427.
Chang, C.T., et al. 1992. Biochem. Int.28, 707.
Weaver, V.M., et al. 1993. Biochem. Cell. Biol.71, 488.
Bourgain, R.H., et al. 1992. Adv. Exp. Med. Biol.316, 427.
Chang, C.T., et al. 1992. Biochem. Int.28, 707.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Oral - Skin Corr. 1B
Storage Class Code
6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Siqi Ming et al.
Frontiers in immunology, 12, 736269-736269 (2021-10-01)
Gastric CD4+T cells contribute to Helicobacter pylori (H. pylori)-induced gastritis by amplifying mucosal inflammation and exacerbating mucosal injuries. However, the pathogenic CD4+ T cell subset involved in gastritis and the potential regulators are still unclear. Here we identified an IL-21-producing
Ming Chang et al.
Bio-protocol, 7(8), e2227-e2227 (2017-04-20)
Arabidopsis pollen is an excellent system for answering important biological questions about the establishment and maintenance of cellular polarity and polar cell growth, because these processes are amenable to the genetic and genomic approaches that are readily available in Arabidopsis.
Chongyuan Luo et al.
Methods in molecular biology (Clifton, N.J.), 1112, 177-193 (2014-01-31)
Genome-wide quantitative profiling of chromatin modifications is a critical experimental approach to study epigenetic and transcriptional control mechanisms. Since first being reported in 2007, chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) has soon became a popular method-of-choice for profiling chromatin
Jordyn Karliner et al.
STAR protocols, 4(1), 101993-101993 (2023-01-06)
Although PC12 cells are a valuable tool in neuroscience research, previously published PC12 cell differentiation techniques fail to consider the variability in differentiation rates between different PC12 cell strains and clonal variants. Here, we present a comprehensive protocol to differentiate
Laïla Giordano et al.
iScience, 25(3), 103877-103877 (2022-03-05)
Malectins from the oligosaccharyltransferase (OST) complex in the endoplasmic reticulum (ER) of animal cells are involved in ER quality control and contribute to the Unfolded Protein Response (UPR). Malectins are not found in plant cells, but malectin-like domains (MLDs) are
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service