324870

Anti-α-Epithelial Cell Sodium Channel (20-42) Rabbit pAb

liquid, Calbiochem^®

• Synonym(s): Anti-α-ENaC
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human, rat
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SCNN1A(6337) ; rat ... Scnn1A(25122)

Description

General description

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~97 kDa glycosylated α-ENaC protein.

Recognizes the ~97 kDa glycosylated α-ENaC protein.

This Anti-α-Epithelial Cell Sodium Channel (20-42) Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation for the detection of α-Epithelial Cell Sodium Channel (20-42).

Immunogen

Human

a synthetic peptide [LMKGNKREEQGLGPEPAAPQQPT(C)] corresponding to amino acids 20-42 of human α-epithelial cell sodium channel (α-ENaC), conjugated to carrier protein

Application

Immunoblotting (1 µg/ml)

Immunoprecipitation (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, 1 mg/ml BSA.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Benos, D.J., and Stanton, B.A. 1999. J. Physiol. London520, 631.
Mano, I., and Driscoll, M. 1999. Bioessays21, 568.
Fyfe, G.K., et al. 1998. Semin. Nephrol.18, 138.

Does not cross-react with β-ENaC or γ-ENaC. Also reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable