1.52007

Chromolith^® RP-18 endcapped

L × I.D. 50 mm x 2 mm HPLC column

• Synonym(s): Chromolith^® FastGradient RP-18 endcapped 50-2 HPLC column
• UNSPSC Code: 41115709

Properties

• material: PEEK column
• Quality Level: 100
• Agency: suitable for USP L1
• product line: Chromolith^®
• feature: endcapped
• packaging: 1 ea of
• extent of labeling: 18% Carbon loading
• parameter: 200 bar pressure; 45 °C max. temp.
• technique(s): LC/MS: suitable; UHPLC-MS: suitable; UHPLC: suitable; mass spectrometry (MS): suitable (High)
• column L × I.D.: 5 cm × 2 mm
• matrix: monolithic silica
• matrix active group: C18 bonding phase
• pore size: 1.5 μm macroporosity; 13 nm mesoporosity
• operating pH range: 2-7.5
• separation technique: reversed phase
• storage temp.: no temp limit

Description

General description

Chromolith^® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith^® FastGradient RP-18 endcapped 50-2 columns have macropores with 1.5 μm diameter, giving a column efficiency exceeding 100,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is octadecylsilane with full end-capping. Chromolith^® FastGradient RP-18 endcapped 50-2 columns fall under the USP L1 Classification. The column efficiency is comparable to a 3-4 μm particle size fully porous particulate column. Chromolith^® FastGradient RP-18 endcapped 50-2 columns are perfectly suitable for LC-MS applications.
The column dimension of 50 mm length and 2 mm I.D. is the best choice for fast separations on any U/HPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enable:

• Rapid separations at very low column back-pressure
• High matrix tolerance, allowing time and cost savings with simplified workflows
• Cost-savings due to extended column lifetimes
• Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith^® RP-18 endcapped 5-2 guard cartridges (3 pieces) [1.52009] with the Chromolith^® Guard Cartridge Holder (kit) 5 mm x 2 mm [1.52004].

The Chromolith^® column coupler can be used to significantly increase the separation efficiency of Chromolith^® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467].

Learn more:
Brochure: Race through your Separations on Any System. Chromolith^® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith^® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Analysis Note

Appearance: passes test
Pressure: ≤ 30 bar
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 100000
Capacity factor (Anthracene): 3.6 - 4.3
Tailing (USP): 0.86 - 1.30 %
Selectivity
separation factor (anthracene/progesterone): 2.30 - 2.60

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany