12-349
Goat Anti-Mouse IgG Antibody, HRP conjugate
Upstate®, from goat
Synonym(s):
Goat IgG Antibody, HRP-Conjugated Goat Anti-Mouse IgG
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46
Recommended Products
biological source
goat
Quality Level
conjugate
peroxidase conjugate
antibody form
purified immunoglobulin
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
mouse
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
shipped in
dry ice
target post-translational modification
unmodified
General description
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Specificity
Recognizes mouse IgG, both heavy and light chains.
Immunogen
Highly purified whole mouse IgG.
Application
Additional Research Applications
ELISA and Immunohistochemistry:
A working dilution of 1:10,000 to 1:40,000 is suggested for ELISA and 1:500 to 1:2,000 for immunohistochemistry.
Optimal antibody dilution for other applications should be determined by the researcher.
ELISA and Immunohistochemistry:
A working dilution of 1:10,000 to 1:40,000 is suggested for ELISA and 1:500 to 1:2,000 for immunohistochemistry.
Optimal antibody dilution for other applications should be determined by the researcher.
Goat Anti-Mouse IgG Antibody, HRP conjugate is an antibody against Mouse IgG for use in ELISA, IH & WB.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Whole Immunoglobulin Secondary Antibodies
Quality
Western Blot Analysis:
Suitable for western blotting (dot blot) at 1:1,000 to 1:4,000 dilution.
Suitable for western blotting (dot blot) at 1:1,000 to 1:4,000 dilution.
Physical form
Purified goat polyclonal IgG in buffer containing conjugated to horseradish peroxidase lyophilized from 0.02 M Potassium Phosphate, 0.15 M NaCl, pH 7.2, 10 mg/mL BSA, and 0.01% gentamicin sulfate.
Rehydration: Add 500 µL sterile, distilled water containing 50% glycerol, to make a 1 mg/mL stock solution.
Rehydration: Add 500 µL sterile, distilled water containing 50% glycerol, to make a 1 mg/mL stock solution.
Storage and Stability
Stable for 1 year at -20ºC from date of receipt.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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