1.00188
Chromocult® RAMBACH™ Agar
ISO 6579, Specialized media for the isolation and differentiation of Salmonella spp. from food and animal feed, water and other materials.
Synonym(s):
Chromogenic Salmonella Agar, Propylene Glycol Deoxycholate Neutral Red Agar, Salmonella Agar
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About This Item
UNSPSC Code:
41106213
NACRES:
NA.74
Agency
ISO 6579
suitable for ISO
Quality Level
form
powder
technique(s)
microbiological culture: suitable
pH
7.3±0.2 (25 °C)
application(s)
food and beverages
storage temp.
15-25°C
suitability
selective for enterobacteriaceae: Salmonella spp.
General description
Chromocult® RAMBACH™ Agar is specifically designed for the differentiation of Salmonella species. The agar is chromogenic, providing two chromogenic substrates – propylene glycol and X-Gal ((5-bromo-4-chloro-3-indoyl- β-D-galactopyranoside).
Certain Salmonella species act on the propylene glycol producing acid which causes the pH indicator in the media to change color, and pinkish-red or crimson colonies appear. Additionally, other members of the family Enterobacteriaceae which are positive for the enzyme β-D-galactosidase cleave the substrate X-Gal and this results in the production of blue-green colonies.
Salmonella species that are positive for the β-D-galactosidase enzyme and can produce acid from propylene glycol appear as purple-violet colonies, while species that are capable of neither appear as yellow or colorless colonies.
Certain Salmonella species act on the propylene glycol producing acid which causes the pH indicator in the media to change color, and pinkish-red or crimson colonies appear. Additionally, other members of the family Enterobacteriaceae which are positive for the enzyme β-D-galactosidase cleave the substrate X-Gal and this results in the production of blue-green colonies.
Salmonella species that are positive for the β-D-galactosidase enzyme and can produce acid from propylene glycol appear as purple-violet colonies, while species that are capable of neither appear as yellow or colorless colonies.
Application
The Chromocult® RAMBACH™ Agar can be used for: -
- The detection of Salmonella in foodstuff, water, animal feed
- The differential diagnosis of Salmonella species in various samples
Analysis Note
- The dehydrated medium appears as dark-green color.
- The prepared medium is opaque and light pink in appearance.
- The pH value at 25 degrees is in the range of 7.1-7.5.
Legal Information
CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
Rambach is a trademark of Balel S.A.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
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