LC/MS (TOF) Analysis of Dermorphin on Ascentis® Express F5
Materiales
Analytical column
CONDITIONS
column
Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)
mobile phase
2 mM ammonium formate (90:10 acetonitrile:water) adjusted to pH 4.5 with formic acid
flow rate
0.4 mL/min
column temp.
35 °C
detector
ESI(+), TIC 100 – 1000 m/z; APCI (+), XIC m/z = 150.1, 203.7, 378.2
injection
1 μL
sample
10 μg/mL in 75:25 acetonitrile:water
Descripción
Analysis Note
Dermorphin is a heptapeptide derived from frogs. It is a painkiller and used illicitly in racehorses so they continue to run through the pain. A rapid analysis of dermorphin standard is shown here on an Ascentis Express F5 column. The HILIC mode separation provided the necessary retention and MS-compatibility, while the pentafluorophenyl (F5) phase chemistry elimated the chelating interactions that are problematic on bare silica.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany