HPLC Analysis of Tricyclic Antidepressants in Serum on Discovery^® C18 after SPE using Discovery^® DSC-18

sample preparation

SPE (Solid Phase Extraction)

SPE tube/cartridge

Discovery DSC-18, 100 mg/1 mL (52602-U)

column

Discovery C18, 15 cm × 4.6 mm, 5 μm preceded by a 2 cm C18 guard column and 0.5 μm frit filter (504955)

mobile phase

[A] acetonitrile: [B] methanol: [C] 25 mM potassium phosphate, monobasic (pH 7 with triethlylamine) (45:25:30, A:B:C)

flow rate

1 mL/min

column temp.

ambient

detector

UV, 254 nm

injection

50 μL, diluted porcine serum extract