HPLC Analysis of Sugars and Taurine in an Energy Drink on Ascentis® Express HILIC using UV and ELSD Detection in Series
CONDITIONS
column
Ascentis Express HILlC, 10 cm x 3.0 mm I.D., 2.7 μm particles (53970-U)
mobile phase
[A] 100 mM ammonium acetate, pH 5.0 with acetic acid; [B] water; [C] acetonitrile; (09:01:90, A:B:C)
flow rate
0.6 mL/min
pressure
812 psi (56 bar)
column temp.
35 °C
detector
ELSD, 55 °C, 3.5 bar nitrogen
injection
2 μL
sample
dilute1:9 in acetonitrile
Descripción
Analysis Note
This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of common drink additives used in caffeinated energy drinks. The analysis was performed with UV and ELSD detectors in series, this chromatogram being from the ELSD.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany