HPLC Analysis of Pyroglutamic Acid Enantiomers on Astec^® CHIROBIOTIC^® T

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] acetonitrile; [B] methanol; [C] acetic acid; [D] triethylamine; (54.5:45:0.3:0.2, A:B:C:D)

flow rate

1 mL/min

column temp.

23 °C

detector

UV, 220 nm

injection

5 μL

sample

1 mg/mL in methanol