HPLC Analysis of mAb 321 on Proteomix^® SCX NP5 versus Competitive SCX using pH and Salt Gradients

column

Proteomix SCX-NP5, 25 cm x 4.6 mm I.D. (PEEK hardware), 5 μm particles (Z777164)

mobile phase

(A) 2.4 mM Tris, 1.5 mM imidazole, 11.6 mM piperazine, pH 6.0; (B) A + 0.5 M NaCl, pH 10.5

flow rate

0.8 mL/min

detector

UV, 280 nm

injection

20 μL

sample

MAb 321 (5 mg/mL)