HPLC Analysis of HSA Fusion Protein on Proteomix^® SAX-NP5

pressure

2016 psi (139 bar)

column temp.

30 °C

column

Proteomix SAX-NP5 25 cm x 4.6mm I.D., 5 μm particles (Z777229)

detector

UV, 280 nm

flow rate

0.5 mL/min

mobile phase

Piperazine, Triethanolamine, bis-tris propane and N-methylpiperazine, 20 mM each,(A) pH 9.7; (B) pH 3.0 ( adjust by HCl )

sample

HSA fusion peptide 5 mg/mL, 10 μL (MW 75 kD, pI 5.0, HSA conjugated peptide in diabetes treatment)