HPLC Analysis of Bovine Serum Albumin (BSA) on Zenix^® SEC-300 versus SRT^® SEC-300, Effect of Particle Size on Resolution

column

SRT SEC-300, 30 cm x 7.8 mm I.D., 5 μm, 300 Å particles (Z777051)

column

Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm, 300 Å particles (Z777033)

mobile phase

150 mM sodium phosphate, pH 7

flow rate

1 mL/min

column temp.

ambient

detector

UV, 214 nm

injection

10 μL

sample

5 mg/mL, BSA