HPLC Analysis of Bisphenol A in Water on Ascentis^® Express C18 after SPE using Supelclean^™ ENVI-18

SPE tube/cartridge

Supelclean ENVI-18, 500 mg/6 mL glass SPE tube (54331-U)

condition

1 mL 1% formic acid in acetonitrile, then 1 mL water

sample

5 mL spiked water blank, concentration of 0.2 μg/mL

elution

2 mL 1% formic acid in acetonitrile

eluate post-treatment

1 mL aliquot of eluent, and evaporate/reconstitute to 0.5 mL in acetonitrile

column

Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)

mobile phase

[A] water; [B] acetonitrile; (60:40, A:B)

flow rate

0.4 mL/min

pressure

3270 psi (256 bar)

column temp.

35 °C

detector

UV, 230 nm

injection

1 μL