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HPLC Analysis of Biogenic Amines on Ascentis® RP-Amide

HPLC Analysis of Biogenic Amines on Ascentis® RP-Amide application for HPLC

Materiales

analytical column

Referencia del producto
Descripción
Precios

565305-U

Ascentis® RP-Amide HPLC Column

5 μm particle size, L × I.D. 15 cm × 2.1 mm

standard

Referencia del producto
Descripción
Precios

S2626

Spermidine

≥99% (GC)

T90344

Tyramine

≥98.0%

55513

Spermine

analytical standard

51799

1,4-Diaminobutano

analytical standard

59964

Histamine

analytical standard

52063

Cadaverine

analytical standard

CONDITIONS

column

Ascentis RP-Amide, 15 cm x 2.1 mm I.D., 5 μm particles (565305-U)

mobile phase

[A] 0.1% ammonium acetate in water (pH unadjusted); [B] acetonitrile

gradient

25 to 75% B in 10 min; held at 75% B for 5 min

flow rate

0.5 mL/min

column temp.

35 °C

detector

UV, 340 nm

injection

10 μL

Descripción

Analysis Note

Following pre-column derivatization of primary amino groups using o-phthaldialdehyde/thiol reagent, the Ascentis RP-Amide was used for the analysis of biogenic amines commonly found in wines. These include histamine, spermine, spermidine, tyramine, putrescine and cadaverine. Structures along with an optimized chromatogram are presented.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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