HPLC Analysis of B-Trichothecenes on Ascentis® Express C18
Materiales
Analytical column
Standard
B-Trichothecene mix, (DON, NIV, 3-AcDON, 15-AcDON)
~100 μg/mL in acetonitrile (each of DON, NIV, 3-AcDON and 15-AcDON), analytical standardCONDITIONS
column
Ascentis Express C18, 10 cm x 3 mm I.D., 2.7 μm particles (53814-U)
mobile phase
[A] water:acetonitrile:methanol (92:4:4); [B] acetonitrile
gradient
0% B held for 5 minutes, to 8% B in 3 minutes, 8% B held for 2 minutes
flow rate
0.8 mL/min
pressure
4061 psi (280 bar)
column temp.
35 °C
detector
UV, 220 nm
injection
40 μL
sample
2 ppm each in water:methanol:acetonitrile (92:4:4)
Descripción
Analysis Note
Mycotoxins are toxic secondary metabolites produced by fungi. Those chemicals exist in food as a result of fungal infection of crops. Mycotoxins have strong resistance to decomposition and digestion, so they remain in the food chain in meat and dairy products. They also resist to temperature, such as cooking and freezing. Their effects on human and animal health include death, cancer, weakened immune systems and as allergens or irritants.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany