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Absorption and fluorescence spectra of polyene antibiotics in the presence of cholesterol.

The Journal of biological chemistry (1992-01-05)
M A Castanho, A Coutinho, M J Prieto
RESUMEN

The alterations in the absorption and fluorescence spectra observed for the polyene antibiotics filipin and nystatin in the presence of cholesterol are due to an exciton interaction (polyene aggregates) and cannot be attributed to a specific sterol-antibiotic complex. Filipin and nystatin molecules partition into the sterol aggregates, these structures being very efficient to induce exciton interaction; the observed splitting profile indicates that the chromophores are in a stacked arrangement (parallel transition dipoles). For filipin incorporated in lipid bilayers, the sterol is able to induce the same type of aggregate, at variance with nystatin.

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Sigma-Aldrich
Filipin III ready made solution, from Streptomyces filipinensis, 1mg/ml in DMSO based solution
Sigma-Aldrich
Filipin complex ready made solution, from Streptomyces filipinensis, 5mg/ml in DMSO based solution