Characterization of ectonucleoside triphosphate diphosphohydrolase (E-NTPDase; EC 3.6.1.5) activity in mouse peritoneal cavity cells.

Characterization of ectonucleoside triphosphate diphosphohydrolase (E-NTPDase; EC 3.6.1.5) activity in mouse peritoneal cavity cells.

Cell biochemistry and function (2017-09-06)

Dhébora Albuquerque Dias, Bruna de Barros Penteado, Lucas Derbocio Dos Santos, Pedro Mendes Dos Santos, Carla Cardozo Pinto Arruda, Maria Rosa Chitolina Schetinger, Daniela Bitencourt Rosa Leal, Jeandre Augusto Dos Santos Jaques

PMID28871607

RESUMEN

This study aimed to characterize the activity of ectonucleoside triphosphate diphosphohydrolase (E-NTPDase; EC 3.6.1.5) in peritoneal cavity cells from BALB/c mice. E-NTPDase was activated in the presence of both calcium (1.5mM) and magnesium (1.5mM) ions. However, the activity was higher in the presence of Ca

MATERIALES

Referencia del producto

Marca

Descripción del producto

A2153

Sigma-Aldrich

Seroalbúmina bovina, lyophilized powder, ≥96% (agarose gel electrophoresis)

75351

Sigma-Aldrich

Oligomicina A, ≥99% (HPLC)

O3125

Sigma-Aldrich

Ouabain octahydrate, ≥95% (HPLC), powder

U4125

Sigma-Aldrich

Uridine 5′-(trihydrogen diphosphate) sodium salt from Saccharomyces cerevisiae, 95-100%

O0200000

Ouabain, European Pharmacopoeia (EP) Reference Standard