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Role of hepatic Annexin A6 in fatty acid-induced lipid droplet formation.

Experimental cell research (2017-07-18)
Rose Cairns, Anna Alvarez-Guaita, Inés Martínez-Saludes, Sundeep J Wason, Jacky Hanh, Shilpa R Nagarajan, Elham Hosseini-Beheshti, Katia Monastyrskaya, Andrew J Hoy, Christa Buechler, Carlos Enrich, Carles Rentero, Thomas Grewal
RESUMEN

Annexin A6 (AnxA6) has been implicated in the regulation of endo-/exocytic pathways, cholesterol transport, and the formation of multifactorial signaling complexes in many different cell types. More recently, AnxA6 has also been linked to triglyceride storage in adipocytes. Here we investigated the potential role of AnxA6 in fatty acid (FA) - induced lipid droplet (LD) formation in hepatocytes. AnxA6 was associated with LD from rat liver and HuH7 hepatocytes. In oleic acid (OA) -loaded HuH7 cells, substantial amounts of AnxA6 bound to LD in a Ca

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Sigma-Aldrich
DL-Glyceraldehyde, ≥90% (GC)
Sigma-Aldrich
Methyl arachidonyl fluorophosphonate, ≥98%, methyl acetate solution (10 mg/mL)