- Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.
Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.
Gene (2014-02-04)
Gonensin O Bozdag, Alaattin Kaya, Ahmet Koc, Gundula A Noll, Dirk Prüfer, Hüseyin Caglar Karakaya
PMID24487090
RESUMEN
Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima and screened it in the yeast system to identify genes that confer resistance to toxic levels of nickel. A cDNA clone (NIC6), which encodes for a putative membrane protein with unknown function, was found to help yeast cells to tolerate toxic levels of nickel. A GFP fused form of Nic6 protein was localized to multivesicular structures in tobacco epidermal cells. Thus, our results suggest a possible role of Nic6 in nickel and intracellular ion homeostasis.
MATERIALES
Referencia del producto
Marca
Descripción del producto
Nickel, pellets, 100g, max. size 10mm, 99.999%
Nickel, foil, 100x100mm, thickness 1.6mm, 99%
Sigma-Aldrich
Nickel, sputtering target, diam. × thickness 2.00 in. × 0.25 in., 99.95% trace metals basis
Nickel, mesh, 100x250mm, nominal aperture 0.73mm, wire diameter 0.25mm, 26x26 wires/inch, open area 55%, plain weave mesh, 99%
Nickel, rod, 100mm, diameter 1.5mm, 99.99+%
Nickel, pellets, 100g, max. size 5mm, 99.99+%
Nickel, sphere, 100pcs, diameter 1.0mm, precision sphere grade 200, 99%
Nickel, foil, 100x100mm, thickness 0.25mm, annealed, 99%
Nickel, foil, 50x50mm, thickness 0.3mm, as rolled, 99.999%
Nickel, mesh, 30x30mm, nominal aperture 0.34mm, thickness 0.025mm, wire diameter 0.041mm, 70 wires/inch, open area 80%, electro-formed mesh, 99.9%
Nickel, foil, 100x100mm, thickness 0.1mm, annealed, 99%
Nickel, pellets, 20g, max. size 5mm, 99.99+%
Nickel, foil, 50x50mm, thickness 1.6mm, 99%
Nickel, wire reel, 100m, diameter 0.025mm, as drawn, 99.98%