Saltar al contenido
MilliporeSigma

Preparation of peach gum polysaccharides using hydrogen peroxide.

Carbohydrate polymers (2013-04-03)
Xin-Cun Yao, Yan Cao, Sai-Kun Pan, Sheng-Jun Wu
RESUMEN

Most polysaccharides cannot dissolve in water but can be hydrolysed using hydrogen peroxide (H2O2) to yield a water-soluble product. This study presents a method of preparing water-soluble polysaccharides from peach gum by hydrolysis using H2O2. Extraction was monitored by the recovery rate. Factors affecting the hydrolysis of peach gum were investigated, and the optimum hydrolysis conditions were determined as follows: time, 8 h; temperature, 55°C; H2O2 concentration, 4% (v/v); and NaOH concentration, 2.0 M. The hydrolysates were filtered, neutralised with HCl, concentrated to ∼20% (w/v), precipitated with 5 volumes of ethanol, freeze-dried, and ground to yield a water soluble and white powder. The polysaccharide content of the product was 97.8%, and the yield was 83.6% (w/w).

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Hidróxido de sodio, ACS reagent, ≥97.0%, pellets
Sigma-Aldrich
Hidróxido de sodio, reagent grade, ≥98%, pellets (anhydrous)
Sigma-Aldrich
Hidróxido de sodio solution, 50% in H2O
Sigma-Aldrich
Hidróxido de sodio solution, BioUltra, for molecular biology, 10 M in H2O
Sigma-Aldrich
Hidróxido de sodio, BioXtra, ≥98% (acidimetric), pellets (anhydrous)
Sigma-Aldrich
Hidróxido de sodio solution, 1.0 N, BioReagent, suitable for cell culture
Sigma-Aldrich
Hidróxido de sodio, puriss., meets analytical specification of Ph. Eur., BP, NF, E524, 98-100.5%, pellets
Sigma-Aldrich
Hidróxido de sodio, reagent grade, 97%, powder
Sigma-Aldrich
Hidróxido de sodio, pellets, semiconductor grade, 99.99% trace metals basis
Sigma-Aldrich
Hidróxido de sodio, puriss. p.a., ACS reagent, reag. Ph. Eur., K ≤0.02%, ≥98%, pellets
Sigma-Aldrich
Hidróxido de sodio solution, 0.1 M
Sigma-Aldrich
Hidróxido de sodio solution, 5.0 M
Sigma-Aldrich
Hidróxido de sodio, puriss. p.a., ACS reagent, K ≤0.02%, ≥98.0% (T), pellets
Sigma-Aldrich
Hidróxido de sodio, beads, 16-60 mesh, reagent grade, 97%
Sigma-Aldrich
Hidróxido de sodio solution, 1 M
Sigma-Aldrich
Ascarite®, Sodium hydroxide-coated silica, 20-30 mesh
Sigma-Aldrich
Hidróxido de sodio, reagent grade, 97%, flakes
Sigma-Aldrich
Hidróxido de sodio, BioUltra, for luminescence, ≥98.0% (T), pellets
Sigma-Aldrich
Ascarite®, Sodium hydroxide-coated silica, 8-20 mesh
Sigma-Aldrich
Hidróxido de sodio, JIS special grade, ≥96.0%
Sigma-Aldrich
Hidróxido de sodio, SAJ first grade, ≥95.0%
Sigma-Aldrich
Hidróxido de sodio, ultra dry, powder or crystals, 99.99% trace metals basis
Sigma-Aldrich
Hidróxido de sodio solution, purum, ≥32%
Supelco
Hidróxido de sodio solution, 0.1 M NaOH in water (0.1N), Eluent concentrate for IC
Sigma-Aldrich
Hidróxido de sodio solution, 6 M
Supelco
Hidróxido de sodio solution, 49-51% in water, eluent for IC
Sigma-Aldrich
Hidróxido de sodio solution, 0.2 M
Sigma-Aldrich
Hidróxido de sodio solution, 0.01 M
Sigma-Aldrich
Hidróxido de sodio solution, 0.05 M
Sigma-Aldrich
Hidróxido de sodio solution, 4 M