- Introducing chemiluminescence resonance energy transfer into immunoassay in a microfluidic format for an improved assay sensitivity.
Introducing chemiluminescence resonance energy transfer into immunoassay in a microfluidic format for an improved assay sensitivity.
Chemical communications (Cambridge, England) (2011-12-03)
Shulin Zhao, Jinwen Liu, Yong Huang, Yi-Ming Liu
PMID22134533
RESUMEN
We report on a novel strategy to improve microfluidic immunoassay sensitivity by introducing chemiluminescence resonance energy transfer (CRET) into the immunoreactions. The proposed CRET-based immunoassay for estradiol (E2, as a model analyte) is one of the most sensitive immunoassay with a limit of detection at 3.6 × 10(-11) M E2 in a microfluidic format.
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