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Total chemical synthesis of photoactivatable proteins for light-controlled manipulation of antigen-antibody interactions.

Chemical science (2016-03-01)
Shan Tang, Zhengpeng Wan, Yiren Gao, Ji-Shen Zheng, Jing Wang, Yan-Yan Si, Xin Chen, Hai Qi, Lei Liu, Wanli Liu
RESUMEN

We report the chemical synthesis of the first photo-activatable protein antigen that can be used to study antigen-antibody interaction mediated responses in B cells. This strategy facilitated fine tuning of the caged protein antigen to optimize its bioactivity and photochemical properties. One optimal molecule, HEL-K96NPE, was totally inert to hen egg lysozyme (HEL)-specific B cells and could only restore its antigenicity upon photoactivation. Combined with real time live cell imaging, the utility of HEL-K96NPE was demonstrated as a proof of concept to quantify B cell synapse formation and calcium influx responses at the single cell level.

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Sigma-Aldrich
Fmoc-L-Lys(Nvoc)-OH, ≥98%