Acidic Western Blot Stripping Solution

Stripping and re-probing a Western blot enables sample conservation and fast correction or adjustments of antibody concentration and incubation time. This recipe is for an acidic Western blot stripping solution that uses glycine hydrochloride and the surfactant sodium dodecyl sulfate (SDS). If the target protein is sensitive to acidic conditions, try the detergent-based stripping solcution recipe.

This recipe calculator enables the accurate preparation of acidic Western blot stripping solution for any milliliter volume. Input your desired volume, click the CALCULATE button, and the table will populate with the amounts of each component needed.

Acidic Western Blot Stripping Solution Recipe Calculator

OTHER RECIPE CALCULATORS FOR WESTERN BLOTTING BUFFERS & SOLUTIONS

Stock Solutions

• 4X SDS Sample Loading Buffer
• 10X Phosphate-Buffered Saline (PBS)
• 10X Tris-Buffered Saline (TBS) Stock Solution
• Stock Components for Western Blot Stripping Solutions

Wash & Blocking Buffers

• 1X Tris-Buffered Saline (TBS) Working Solution
• 1X Tris-Buffered Saline, 0.1% Tween® 20 Detergent (TBST)
• 1X PBS (Phosphate-Buffered Saline)
• 1X Phosphate-Buffered Saline, 0.1% Tween® 20 Detergent (PBST)
• Non-Fat Dry Milk (NFDM) Blocking Buffer
• Bovine Serum Albumin (BSA) Blocking Buffer

Stripping Solutions

• Detergent-based Western Blot Stripping Buffer
• Stock Components for Detergent-based Western Blot Stripping Solutions