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CellASIC® ONIX2 Microfluidics

Microfluidic platform for precise control of cell culture environment for advanced live-cell imaging and microscopy.

The ability to grow, observe and manipulate complex cell cultures requires precision control over the cell culture environment. The second-generation CellASIC® ONIX2 Microfluidic System is a powerful, automated platform for precise manipulation of multiple cell culture parameters, such as gas mixture, temperature, and media, buffer, and treatment flow.

CellASIC® ONIX2 Live-Cell Imaging Platform

The CellASIC® ONIX2 Microfludic System transforms your existing microscope into an automated system for advanced live-cell imaging and analysis.
System components:

  • Small footprint, integrated microincubator controller maintains culture environment
  • Optically clear microfluidic plates allow live-cell imaging for many applications
  • Low-profile culture chambers and manifold mount easily on an inverted microscope
  • Intuitive software allows easy setup for truly automated hands-free cell culture
Related Product Literature
CellASIC® ONIX2 Microfluidic System
CellASIC® ONIX microfluidic

CellASIC® ONIX2 Microfluidic Plates

The CellASIC® ONIX2 microfluidic cell culture plates are designed for optimal cell health, while allowing dynamic live-cell studies with mammalian cells, bacterial cells, and yeast cells.

Key plate features:

  • Microfluidic volumes allow fast perturbations and exchanges of environmental fluids
  • Perfusion microbarriers allow continuous reagent transport without cell stress
  • Optical glass bottom and chamber enables high-resolution live-cell imaging
  • Flexible design allows multiple independent experiments simultaneously
  • Cells have continuous access to nutrients and removal of waste is uninterrupted

Key Applications for the ONIX Live Cell Imaging and Analysis System

Diverse application-specific plate designs give you the flexibility to conduct the culture and assays that yield the most relevant data. Plates are designed to keep both adherent and suspension cells in focus, and are optimized for cells of varying diameters, including mammalian, yeast, and bacterial cells.
Popular applications of the CellASIC® ONIX2 platform include:

  • Microscopic analysis of 3D cell and organoid culture
  • Chemotaxis/migration in response to chemogradients
  • Real-time and dynamic cellular response to compound addition/withdrawal and changing environmental conditions
  • Primary neuron culture over 21 days
  • Host-pathogen interactions
  • Bacterial single-cell response and biofilm formation
Related Product Categories
Live Cell Imaging Reagents
Reagents for live cell imaging are designed to be nontoxic and to protect live cells while optimizing visualization during real-time studies of cell trafficking and localization, cell health, gene expression, migration, cytoskeletal dynamics, and other cellular events.
Mammalian Cell Lines
Cell lines from human and other mammalian species are essential for modeling disease and biological systems, and are critical tools for protein, antibody, virus, and vaccine production. We offer authenticated, contaminant-free cell lines, many in partnership with ECACC.
Primary Cells
Primary cells are derived directly from living tissue and closely replicate the physiology of biological systems. Our collection includes blood, patient tumor xenografts, and hepatocytes essential for drug screening and ADME/Tox studies.
Stem Cells
Our complete range of ES and iPS cell-qualified products includes stem cell lines and media for differentiation and maturation, and provides stem cell researchers with convenient and cost-effective solutions for the reliable culture of pluripotent stem cells.
Related Applications
Imaging Analysis & Live Cell Imaging
An overview of current tools and reagents for live cell imaging and analysis with applications in cell trafficking, cell health, gene expression analysis, cell migration, 3D cell culture, cytoskeletal dynamics and more.
Mammalian Cell Culture
Applications, principles, and methods for in vitro mammalian cell culture including links to articles, protocols, and videos for proper aseptic technique, passaging and subculturing, and culture media considerations.
Primary Cell Culture
Primary cells isolated directly from human and animal sources maintain physiological relevance of the tissues from which they are derived, and are used for ADME/Tox screening. Find applications and methods for culturing mammalian primary cells in vitro.
Stem Cell Culture
An overview of different stem cell types, stem cell culture basics and stem cell applications in basic research and clinical research applications, such as tissue regeneration, genetic diseases and cancer.
3D Cell Culture
Applications, principles, and techniques for three-dimensional (3D) cell culture. Includes techniques and descriptions of scaffold and scaffold-free methods matrices for the culture of complex in vitro models including organoids and spheroids.
Microbial Cell Culture
An overview of microbiological cell culture and its applications in molecular biology, cloning, and recombinant protein expression as well as cultivation for the isolation, differentiation, and identification of disease-causing microbes in clinical researc
Cell Growth & Maintenance
A synopsis of the conditions, media, nutrients, cultureware, and environments required for successful propagation of mammalian and yeast cells. Nutrient reagents, sterile technique and filtration, and requirements for 2D, 3D, and suspension cultures are described.

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