Biomacromolecule Analysis By Multiple Modes of Chromatography

WEBINAR

The separation and characterization of biomacromolecules presents a distinct analytical challenge due to the intricate nature of the analytes. This complexity arises from the numerous modifications to these analytes in each sample, including phosphorylation, methylation, and glycosylation, among others. The heterogeneity of individual analytes, combined with a biomolecule's tendency to interact with silica-based stationary phases, can lead to significant broadening and tailing of chromatographic peaks. Additionally, conventional electrospray ionization mass spectrometers may not effectively ionize large proteins. Furthermore, poorly separated protein mixtures can result in further ion suppression. Consequently, the demand for highly efficient, robust, and reproducible analytical methods is not merely a suggestion but a necessity for the accurate characterization of proteinaceous samples.

This seminar will concentrate on strategies and techniques to enhance efficiency and resolution when employing various chromatography modes to analyze biomacromolecules. It will delve into method development, troubleshooting aspects, and chromatographic considerations for biomacromolecule characterization suitable for mass spectrometry (MS) and Multi-Angle Light Scattering (MALS) detection platforms.  By the end of the seminar, participants will gain insight into:

• Selecting and understanding the importance of phase chemistry and particle morphology in biomolecule analysis
• Best practices for selecting appropriate mobile phase systems for each chromatography mode
• Diagnosing the root causes of issues encountered when troubleshooting biomolecule separations