CWA-1171

Anti-Human LIF-R (1C7) ColorWheel^® Dye-Ready mAb

• Synonym(s): Leukemia inhibitory factor receptor;LIF receptor;LIF-R; D-factor/LIF receptor;CD118
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1C7, monoclonal
• product line: ColorWheel^®
• form: lyophilized
• purified by: using protein G
• species reactivity: human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• technique(s): flow cytometry: suitable
• isotype: IgG1κ
• epitope sequence: Extracellular domain
• Protein ID accession no.: P42702.1
• UniProt accession no.: P42702
• compatibility: for use with ColorWheel^® Dyes (Required, (Sold Separately))
• greener alternative category: Aligned,
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... Lifr(3977)

Description

General description

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Specificity

Clone 1C7 is a mouse monoclonal antibody that detects LIF-R (CD118). It targets an epitope within the extracellular domain.

Immunogen

Recombinant protein fragment corresponding to amino acids 2-538 of human LIF-R, human IgG Fc fusion protein.

Application

Quality Control Testing

Evaluated by Flow Cytometry in CHO cells stably transfected with D1IgD2-mycDAF.

Flow Cytometry Analysis: Staining of one million Staining of one million CHO cells stably transfected with D1IgD2-mycDAF was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1171, Anti-Human LIF-R (1C7) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Tested Applications

Flow Cytometry Analysis: Staining of one million Staining of one million CHO cells stably transfected with D1IgD2-mycDAF was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1171, Anti-Human LIF-R (1C7) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or a 1:1 mixture of an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Compatibility

Requires ColorWheel^® Dye (Sold Separately)

Target description

Leukemia inhibitory factor receptor (UniProt: P42702; also known as LIF receptor, LIF-R, CD118) is encoded by the Lifr gene (Gene ID: 3977) in human. Leukemia inhibitory factor (LIF) mediates its biological actions via binding to its high affinity receptor made of the low-affinity LIF receptor subunit gp190 (LIF-R) and the gp130 subunit. LIF-R, a member of the type I cytokine receptor family, is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-44), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 45-833), a transmembrane domain (aa 834-858), and a cytoplasmic domain (aa 859-1097). It also contains six fibronectin type III domains. It contains a WSXWS motif (aa 519-523) that is shown to be essential for proper protein folding and cell-surface receptor binding. Its box 1 motif (aa 869-877) is required for JAK interaction and activation. A secreted form (isoform 2) has also been described that inhibits the biological activity of LIF by blocking its binding to receptors on target cells. LIF-R forms a heterodimer with interleukin 6 cytokine family signal transducer (IL6ST) and this heterodimer interacts with the complex formed by ciliary neurotrophic factor (CNTF) and CNTF receptor (CNTFR). Over-expression of LIF and LIF-R has been reported in many solid cancers. LIF/LIF-R activate oncogenic signaling pathways, including JAK/STAT3, MAPK, Akt, and mTOR. This signaling also plays a role in modulating multiple immune cell types present in tumor microenvironment. This ColorWheel^® Dye-Ready monoclonal antibody was developed using our Cat. No. MABD150; Anti-LIF Receptor, clone 1C7. (Ref.: Viswanadhapalli, S., et al (2022). Genes Dis. 9(4); 973-980; Hisaka, T., et al. (2004). Comp. Hepatol.3(1); 10; Taupin, J-L., et al. (2001). J. Biol. Chem. 276(51); 47975-47981).

Physical form

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitution

1.0 mg/mL after reconstitution at 25 µL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 µL PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable