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Merck

LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5 (Monitored Ions)

LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5 (Monitored Ions) application for HPLC

材料

分析柱

產品號碼
描述
訂價

Ascentis® Express F5, 2.7 μm HPLC 色谱柱

2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

標準

產品號碼
描述
訂價

1α-Hydroxyvitamin D3

≥97.0% (HPLC)

3--25-羟基维生素 D3

98% (CP)

3--25-羟基维生素 D2 溶液

100 μg/mL in ethanol, 98% (CP)

25-羟基维生素D2标准液 CRM 溶液

50 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

25-羟基维生素D3标准液 CRM 溶液

100 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

1α,25-二羟基维生素D3标准液 CRM 溶液

5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

1α,25-二羟基维生素D2标准液 溶液

5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

流動相成分

產品號碼
描述
訂價

甲酸铵

eluent additive for LC-MS, LiChropur, ≥99.0%

CONDITIONS

column

Ascentis Express  F5, 15 cm x 2.1 mm I.D., 2.7 μm particles (53571-U)

mobile phase

[A] 5 mM ammonium formate water; [B] methanol; (25:75, A:B)

flow rate

0.4 mL/min

pressure

5178 psi (357 bar)

column temp.

40 °C

detector

ESI(+), 100-1000 m/z

injection

2 μL, each compound 300 ng/mL in 40:60 (water:methanol)

描述

分析報告

The metabolic role and health implications of the various vitamin D isoforms are of current clinical interest. Therefore, it is important to have an analytical method that will resolve all of the known isoforms with necessary sensitivity and specificity.This application shows that a combination of Ascentis Express F5 with MS detection provided rapid resolution of seven such compounds. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany