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Merck

LC/MS Analysis of Erythromycin Analogs on Ascentis® Express HILIC

LC/MS Analysis of Erythromycin Analogs on Ascentis® Express HILIC application for HPLC

材料

分析柱

產品號碼
描述
訂價

53981-U

Ascentis® Express HILIC, 2.7 μm HPLC色谱柱

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

標準品

產品號碼
描述
訂價

E8755

乙琥红霉素

45674

红霉素

tested according to Ph. Eur.

1242021

Erythromycin C

United States Pharmacopeia (USP) Reference Standard

流動相成分

產品號碼
描述
訂價

70221

甲酸铵

eluent additive for LC-MS, LiChropur™, ≥99.0%

CONDITIONS

column

Ascentis Express HILlC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)

mobile phase

[A] 100 mM ammonium formate, pH 3.0 (titrated with formic acid); [B] acetonitrile; (10:90, A:B)

flow rate

1.0 mL/min

pressure

1247 psi (86 bar)

column temp.

35 °C

detector

ESI(+); SIR

injection

5 μL

描述

分析報告

This application shows the suitability of Ascentis Express Silica columns for the analysis of erythromycin analogs in aqueous normal phase/HILIC mode of chromatography. Other relatively hydrophobic aminoglycosides have also been shown to separate under these conditions.

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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