HPLC Analysis of Vitamins, Water Soluble on Discovery^® C18

column

Discovery C18, 15 cm × 4.6 mm I.D., 5 μm particles (504955)

mobile phase

[A] 50 mM potassium phosphate, dibasic, pH 4.5 to methanol: [B] 50mM potassium phosphate, dibasic, pH 4.0 (30:70, A:B) in 8 min

flow rate

1 mL/min

column temp.

ambient

detector

UV, 254 nm

injection

1 μL