跳轉至內容
Merck

HPLC Analysis of Triacylglycerols in Olive Oil on Discovery® HS C18 after Sample Prep using Discovery® DSC-Si (Propionitrile Mobile Phase, ELSD Detection)

HPLC Analysis of Triacylglycerols in Olive Oil on Discovery® HS C18 after Sample Prep using Discovery® DSC-Si (Propionitrile Mobile Phase, ELSD Detection) application for HPLC

材料

SPE管或板

產品號碼
描述
訂價

Discovery® DSC-Si SPE 小柱

bed wt. 1 g, volume 6 mL, pk of 30

分析柱

產品號碼
描述
訂價

Discovery® HS C18 高效液相色谱柱

5 μm particle size, L × I.D. 25 cm × 4.6 mm

標準

產品號碼
描述
訂價

甘油三油酸酯

≥99%

三亚油酸甘油酯

≥98% (TLC), liquid

橄榄油

analytical standard

流動相成分

產品號碼
描述
訂價

丙腈

99%

CONDITIONS

SPE tube/cartridge

Discovery DSC-Si, 1 gm/6 mL (52656-U)

condition

6 mL hexane

sample addition

0.12 g oil in 0.5 mL hexane

elution

10 mL hexane-diethyl ether 87-13 (v/v)

eluate post-treatment

evaporation to dryness, reconstituted into 2 mL acetone

column

Discovery HS C18, 25 cm x 4.6 mm I.D., 5 μm particles (568523-U)

mobile phase

propionitrile

flow rate

1.5 mL/min

pressure

1117 psi (77 bar)

column temp.

30 °C

detector

ELSD

injection

5 μL

描述

分析報告

Olive oil was pre-cleaned using Silica SPE, analyzed as 5% sample in acetone (w/v).

法律資訊

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany