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Merck

HPLC Analysis of Steroids on Ascentis® Express C18 (10 cm x 2.1 mm I.D.)

HPLC Analysis of Steroids on Ascentis® Express C18 (10 cm x 2.1 mm I.D.) application for HPLC

材料

分析柱

產品號碼
描述
訂價

53823-U

Ascentis® Express C18, 2.7 μm HPLC 色谱柱

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

標準品

產品號碼
描述
訂價

T1500

睾酮

≥98%

E-058

表睾酮 溶液

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

M-912

美雄酮 溶液

1.0 mg/mL in 1,2-dimethoxyethane, ampule of 1 mL, certified reference material, Cerilliant®

N-050

诺龙 溶液

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

N-914

Norethandrolone solution

1.0 mg/mL in 1,2-dimethoxyethane, ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)

mobile phase

[A] water; [B] acetonitrile; (60:40, A:B)

flow rate

0.2 mL/min

pressure

2944 psi (203 bar)

column temp.

35 °C

detector

UV, 254 nm

injection

2 μL

sample

50 mg/L in 75:25, water:methanol

描述

分析報告

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of 5 steroids.

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

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