HPLC Analysis of Carotene Compounds on Ascentis^® RP-Amide

column

Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)

mobile phase

100% acetonitrile

flow rate

1.0 mL/min

column temp.

35 °C

detector

MS, ESI(+) in selective ion monitoring (SIR) mode

injection

5 μL

sample

10 μg/mL each in 100% acetonitrile