HPLC Analysis of an Energy Drink on Ascentis^® Express HILIC, 10 cm (UV Detection)

column

Ascentis Express HILIC, 5 cm x 3 mm I.D., 2.7 μm particles (53967-U)

mobile phase

[A] 100 mM ammonium acetate, pH 5 (titrated with acetic acid); [B] water; [C] acetonitrile; (9:1:90, A:B:C:)

flow rate

0.6 mL/min

column temp.

35 °C

detector

UV, 254 nm

injection

2 μL

sample

Energy Drink Diluted 1:9 in acetonitrile