HPLC Analysis of Barbiturates on Discovery^® C18

column

Discovery C18, 15 cm × 4.6 mm I.D., 5 μm particles (504955)

mobile phase

[A] acetonitrile; [B] 25 mM potassium phosphate buffer, pH 6.9 (25:75, A:B)

flow rate

1.5 mL/min

column temp.

ambient

detector

UV, 254 nm

injection

25 μL