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GC Analysis of Pesticides in Fruits and Vegetables on a 14% Cyanopropylphenyl Column after SPE using Supelclean ENVI-Carb

GC Analysis of Pesticides in Fruits and Vegetables on a 14% Cyanopropylphenyl Column after SPE using Supelclean ENVI-Carb™ suitable for GC, application for SPE

材料

SPE管或板

產品號碼
描述
訂價

Supelclean ENVI-Carb SPE 管

bed wt. 500 mg, volume 6 mL, pk of 30

分析柱

產品號碼
描述
訂價

Equity -1701 毛细管 GC 色谱柱

L × I.D. 30 m × 0.25 mm, df 0.25 μm

應用

產品號碼
描述
訂價

HPLC Analysis of Pesticides on Ascentis® C8 (15 cm x 4.6 mm x 5 μm)

application for HPLC

CONDITIONS

sample preparation

SPE (Solid Phase Extraction)

sample/matrix

extract from 50 g homogenized fruit or vegetable sample

SPE tube/cartridge

ENVI-Carb, 500 mg/6 mL (57094)

sample addition

0.5 mL concentrated sample extract

elution

20 mL acetonitrile:toluene (3:1)

eluate post-treatment

Concentrate to 2 mL by rotary evaporation. Add 2 x 10 mL acetone concentrating to 2 mL after each acetone addition. (Add 50 μL I.S. (500 ng/μL cis-chlordane in acetone) then dilute to 2.5 mL with acetone.)

column

14% cyanopropylphenyl polysiloxane, 30m × 0.25 mm I.D., 0.15 μm (Supelco equivalent, Equity-1701, 28372-U)

oven

70 °C (hold 2 min) to 130 °C at 25 °C; 130 °C to 220 °C at 2 °C/min; 220 °C to 280 °C at 10 °C/min and hold 4.6 min

detector

MSD, 285 °C

carrier gas

helium

injection

2 μL, splitless

描述

應用

Sample Pre-treatment:
Homogenize 50 g chopped fruit/vegetable sample in 100 mL acetonitrile. Add 10 g sodium chloride and homogenize 5 min. Transfer 13 mL of acetonitrile layer to clean centrifuge tube. Centrifuge at high speed for 5 min. Evaporate 10 mL aliquot of supernatant to 0.5 mL under Nitrogen at 35°C.

法律資訊

ENVI-Carb is a trademark of Sigma-Aldrich Co. LLC