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Merck
  • Glycolysis inhibition improves photodynamic therapy response rates for equine sarcoids.

Glycolysis inhibition improves photodynamic therapy response rates for equine sarcoids.

Veterinary and comparative oncology (2017-01-28)
J P Golding, J G Kemp-Symonds, J M Dobson
摘要

Photodynamic therapy (PDT) holds great promise in treating veterinary and human dermatological neoplasms, including equine sarcoids, but is currently hindered by the amount of photosensitiser and light that can be delivered to lesions thicker than around 2 mm, and by the intrinsic antioxidant defences of tumour cells. We have developed a new PDT technique that combines an efficient transdermal penetration enhancer solution, for topical delivery of 5-aminolevulinic acid (ALA) photosensitiser, with acute topical post-PDT application of the glycolysis inhibitor lonidamine. We show that the new PDT combination treatment selectively kills sarcoid cells in vitro, with repeated rounds of treatment increasing sarcoid sensitisation to PDT. In vivo, ALA PDT followed by 600 μM lonidamine substantially improves treatment outcomes for occult, verrucous, nodular and fibroblastic sarcoids after 1 month (93% treatment response in 27 sarcoids), compared with PDT using only ALA (14% treatment response in 7 sarcoids).

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Sigma-Aldrich
D-2-脱氧葡萄糖, ≥98% (GC), crystalline
Sigma-Aldrich
Span® 20
Sigma-Aldrich
5-氨基乙酰丙酸盐 盐酸盐, BioReagent, suitable for cell culture, powder, ≥98%
Sigma-Aldrich
氯尼达明, mitochondrial hexokinase inhibitor