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Mixed alkyl aryl phosphonate esters as quenched fluorescent activity-based probes for serine proteases.

Mixed alkyl aryl phosphonate esters as quenched fluorescent activity-based probes for serine proteases.

Organic & biomolecular chemistry (2015-01-03)

Sevnur Serim, Philipp Baer, Steven H L Verhelst

摘要

Activity-based probes (ABPs) are powerful tools for the analysis of active enzyme species in whole proteomes, cells or animals. Quenched fluorescent ABPs (qABPs) can be applied for real time imaging, allowing the visualization of dynamic enzyme activation by fluorescent microscopy. Unfortunately, qABPs are only available for a few enzymes. We here describe the design and synthesis of qABPs for serine proteases based on a phosphonate ester scaffold.

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乙酸, glacial, ACS reagent, ≥99.7%

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乙酸, glacial, ReagentPlus^®, ≥99%

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硫酸镁, anhydrous, ReagentPlus^®, ≥99.5%

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2-丁酮, ACS reagent, ≥99.0%

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乙酸, glacial, ≥99.99% trace metals basis

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乙酸溶液, suitable for HPLC

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(+)-抗坏血酸钠 L , crystalline, ≥98%

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2-丁酮, suitable for HPLC, ≥99.7%

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溴化锂, ReagentPlus^®, ≥99%

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