跳轉至內容
Merck
  • Towards the fabrication of artificial 3D microdevices for neural cell networks.

Towards the fabrication of artificial 3D microdevices for neural cell networks.

Biomedical microdevices (2015-02-15)
Andrew A Gill, Ílida Ortega, Stephen Kelly, Frederik Claeyssens
摘要

This work reports first steps towards the development of artificial neural stem cell microenvironments for the control and assessment of neural stem cell behaviour. Stem cells have been shown to be found in specific, supportive microenvironments (niches) and are believed to play an important role in tissue regeneration mechanisms. These environments are intricate spaces with chemical and biological features. Here we present work towards the development of physically defined microdevices in which neural and neural stem cells can be studied in 3-dimensions. We have approached this challenge by creating bespoke, microstructured polymer environments using both 2-photon polymerisation and soft lithography techniques. Specifically, we have designed and fabricated biodegradable microwell-shaped devices using an in house synthetized polymer (4-arm photocurable poly-lactid acid) on a bespoke 2-photon polymerisation (2PP) set-up. We have studied swelling and degradation of the constructs as well as biocompatibility. Moreover, we have explored the potential of these constructs as artificial neural cell substrates by culturing NG108-15 cells (mouse neuroblastoma; rat glioma hybrid) and human neural progenitor cells on the microstructures. Finally, we have studied the effects of our artificial microenvironments upon neurite length and cell density.

材料
產品編號
品牌
產品描述

Sigma-Aldrich
氯仿, contains 100-200 ppm amylenes as stabilizer, ≥99.5%
庫存單位
包裝尺寸
供貨能力
價格
數量
Sigma-Aldrich
氯仿, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains amylenes as stabilizer
庫存單位
包裝尺寸
供貨能力
價格
數量
Sigma-Aldrich
氯仿, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
庫存單位
包裝尺寸
供貨能力
價格
數量
Sigma-Aldrich
氯仿, contains ethanol as stabilizer, ACS reagent, ≥99.8%
庫存單位
包裝尺寸
供貨能力
價格
數量
Sigma-Aldrich
氯仿, suitable for HPLC, ≥99.8%, amylene stabilized
庫存單位
包裝尺寸
供貨能力
價格
數量
Sigma-Aldrich
3-(三甲氧基甲硅基)甲基丙烯酸丙酯, 98%
庫存單位
包裝尺寸
供貨能力
價格
數量
Sigma-Aldrich
氯仿, puriss. p.a., reag. ISO, reag. Ph. Eur., 99.0-99.4% (GC)
庫存單位
包裝尺寸
供貨能力
價格
數量
Sigma-Aldrich
氯仿, contains amylenes as stabilizer, ACS reagent, ≥99.8%
庫存單位
包裝尺寸
供貨能力
價格
數量
Sigma-Aldrich
氯仿, ReagentPlus®, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
庫存單位
包裝尺寸
供貨能力
價格
數量
Sigma-Aldrich
氯仿, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains 0.5-1.0% ethanol as stabilizer
庫存單位
包裝尺寸
供貨能力
價格
數量
Sigma-Aldrich
2-甲基-2-丁烯, ≥95.0% (GC)
庫存單位
包裝尺寸
供貨能力
價格
數量
Sigma-Aldrich
氯仿, ACS spectrophotometric grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
庫存單位
包裝尺寸
供貨能力
價格
數量
Sigma-Aldrich
3-(三甲氧基甲硅基)甲基丙烯酸丙酯, ≥97%
庫存單位
包裝尺寸
供貨能力
價格
數量
Sigma-Aldrich
氯仿, biotech. grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
庫存單位
包裝尺寸
供貨能力
價格
數量
Sigma-Aldrich
氯仿, contains ethanol as stabilizer, meets analytical specification of BP, 99-99.4% (GC)
庫存單位
包裝尺寸
供貨能力
價格
數量
Sigma-Aldrich
氯仿, ≥99%, PCR Reagent, contains amylenes as stabilizer
庫存單位
包裝尺寸
供貨能力
價格
數量
Sigma-Aldrich
氯仿, anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer
庫存單位
包裝尺寸
供貨能力
價格
數量
Supelco
氯仿, analytical standard
庫存單位
包裝尺寸
供貨能力
價格
數量
Supelco
氯仿, Pharmaceutical Secondary Standard; Certified Reference Material
庫存單位
包裝尺寸
供貨能力
價格
數量
Sigma-Aldrich
氯仿, anhydrous, contains amylenes as stabilizer, ≥99%
庫存單位
包裝尺寸
供貨能力
價格
數量
Supelco
残留溶剂-氯仿, Pharmaceutical Secondary Standard; Certified Reference Material
庫存單位
包裝尺寸
供貨能力
價格
數量
Sigma-Aldrich
2-甲基-2-丁烯, ≥99%, purified by redistillation
庫存單位
包裝尺寸
供貨能力
價格
數量
Supelco
2-甲基-2-丁烯, analytical standard
庫存單位
包裝尺寸
供貨能力
價格
數量
Supelco
氯仿, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
庫存單位
包裝尺寸
供貨能力
價格
數量
Sigma-Aldrich
氯仿, ACS reagent, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
庫存單位
包裝尺寸
供貨能力
價格
數量
Sigma-Aldrich
2-甲基-2-丁烯, technical grade, 90%
庫存單位
包裝尺寸
供貨能力
價格
數量
Sigma-Aldrich
氯仿, ACS reagent, ≥99.8%, contains amylenes as stabilizer
庫存單位
包裝尺寸
供貨能力
價格
數量