跳轉至內容
Merck
  • Epigenetic regulation of Atrophin1 by lysine-specific demethylase 1 is required for cortical progenitor maintenance.

Epigenetic regulation of Atrophin1 by lysine-specific demethylase 1 is required for cortical progenitor maintenance.

Nature communications (2014-12-19)
Feng Zhang, Dan Xu, Ling Yuan, Yiming Sun, Zhiheng Xu
摘要

Lysine-specific demethylase 1 (LSD1) is involved in gene regulation and development; however, its precise function, molecular targets and underlying mechanisms during development are poorly understood. Here we show that LSD1 is required for neuronal progenitor cell (NPC) maintenance during cortical development. A ChIP-seq analysis identified a LSD1-binding site (LBAL) downstream of Atrophin1 (ATN1). Surprisingly, tranylcypromine (LSD1 inhibitor) treatment increased H3K4 methylation at LBAL, leading to ATN1 repression and NPC differentiation. Knockdown of LSD1 and ATN1 phenocopied each other in inducing NPC premature differentiation and depletion, which could be rescued by ATN1 overexpression, suggesting that LSD1 controls NPC differentiation via regulation of ATN1 methylation status and expression. The involvement of LSD1 in ATN1 expression and NPC maintenance were confirmed in knockout mice. These findings hint at the potential application for the clinical drug, tranylcypromine, in the prevention and/or treatment of ATN1-associated degenerative disease, dentatorubral-pallidoluysian atrophy.

材料
產品編號
品牌
產品描述

Sigma-Aldrich
纯乙醇, 200 proof, for molecular biology
Sigma-Aldrich
2-丙醇, suitable for HPLC, 99.9%
Sigma-Aldrich
2-丙醇, ACS reagent, ≥99.5%
Sigma-Aldrich
纯乙醇, 200 proof, ACS reagent, ≥99.5%
Sigma-Aldrich
纯乙醇, 200 proof, HPLC/spectrophotometric grade
Sigma-Aldrich
纯乙醇, 200 proof, meets USP testing specifications
Sigma-Aldrich
纯乙醇, 190 proof, for molecular biology
Sigma-Aldrich
2-丙醇, HPLC Plus, for HPLC, GC, and residue analysis, 99.9%
Sigma-Aldrich
异丙醇, meets USP testing specifications
Sigma-Aldrich
纯乙醇, 200 proof, anhydrous, ≥99.5%
Sigma-Aldrich
2-丙醇, for molecular biology, BioReagent, ≥99.5%
Sigma-Aldrich
2-丙醇, Laboratory Reagent, ≥99.5%
Sigma-Aldrich
2-丙醇, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8% (GC)
Sigma-Aldrich
酒精, BioUltra, for molecular biology, ≥99.8%, (absolute alcohol, without additive, A15 o1)
Sigma-Aldrich
酒精, ACS reagent, prima fine spirit, without additive, F15 o1
Sigma-Aldrich
酒精, purum, absolute ethanol, denaturated with 4.8% isopropanol, A15 IPA1, ≥99.8% (based on denaturant-free substance)
Sigma-Aldrich
异丙醇, ≥99.7%, FCC, FG
Sigma-Aldrich
2-丙醇, anhydrous, 99.5%
Sigma-Aldrich
2-丙醇, BioUltra, for molecular biology, ≥99.5% (GC)
Sigma-Aldrich
2-丙醇, puriss. p.a., ACS reagent, ≥99.8% (GC)
Sigma-Aldrich
2-丙醇, electronic grade, 99.999% trace metals basis
Sigma-Aldrich
纯乙醇, 190 proof, ACS spectrophotometric grade, 95.0%
Sigma-Aldrich
纯乙醇, 190 proof, meets USP testing specifications
Supelco
酒精, standard for GC
Sigma-Aldrich
酒精, purum, fine spirit, denaturated with 4.8% methanol, F25 METHYL1, ~96% (based on denaturant-free substance)
Sigma-Aldrich
2-丙醇, puriss., meets analytical specification of Ph. Eur., BP, USP, ≥99.5% (GC)
Supelco
2-丙醇, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
无水乙醇, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
酒精, for residue analysis