- A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation.
A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation.
Nature chemical biology (2013-12-10)
Jianzhao Liu, Yanan Yue, Dali Han, Xiao Wang, Ye Fu, Liang Zhang, Guifang Jia, Miao Yu, Zhike Lu, Xin Deng, Qing Dai, Weizhong Chen, Chuan He
PMID24316715
摘要
N(6)-methyladenosine (m(6)A) is the most prevalent and reversible internal modification in mammalian messenger and noncoding RNAs. We report here that human methyltransferase-like 14 (METTL14) catalyzes m(6)A RNA methylation. Together with METTL3, the only previously known m(6)A methyltransferase, these two proteins form a stable heterodimer core complex of METTL3-METTL14 that functions in cellular m(6)A deposition on mammalian nuclear RNAs. WTAP, a mammalian splicing factor, can interact with this complex and affect this methylation.