跳轉至內容
Merck
  • The Membrane Protein Sortilin Can Be Targeted to Inhibit Pancreatic Cancer Cell Invasion.

The Membrane Protein Sortilin Can Be Targeted to Inhibit Pancreatic Cancer Cell Invasion.

The American journal of pathology (2020-06-12)
Fangfang Gao, Nathan Griffin, Sam Faulkner, Xiang Li, Simon J King, Phillip Jobling, Jim W Denham, Chen Chen Jiang, Hubert Hondermarck
摘要

Pancreatic cancer has a dismal prognosis, and there is no targeted therapy against this malignancy. The neuronal membrane protein sortilin is emerging as a regulator of cancer cell development, but its expression and impact in pancreatic cancer are unknown. This study found that sortilin expression was higher in pancreatic cell lines versus normal pancreatic ductal epithelial cells, as shown by Western blot analysis and mass spectrometry. The increased sortilin level in pancreatic cancer cells was confirmed by immunohistochemistry in a series of 99 human pancreatic adenocarcinomas versus 48 normal pancreatic tissues (P = 0.0014). Sortilin inhibition by siRNA and the pharmacologic inhibitor AF38469 strongly reduced the adhesion and invasion of pancreatic cancer cells without affecting cell survival and viability. Sortilin inhibition also decreased the phosphorylation of the focal adhesion kinase in Tyr925. Together, these data show that sortilin contributes to pancreatic cancer invasion and could eventually be targeted in therapy.

材料
產品編號
品牌
產品描述

Sigma-Aldrich
泰莫西芬, ≥99%
Sigma-Aldrich
β-雌二醇, ≥98%
Sigma-Aldrich
单克隆抗 β-肌动蛋白抗体 小鼠抗, clone AC-74, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
QCM ECMatrix细胞侵袭试验,24孔(8 µm),比色法, The CHEMICON Cell Invasion Assay Kit uses a 24-well plate, with 8 um pores, which provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.
Sigma-Aldrich
二氯甲基二乙胺 盐酸盐, 98%