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Merck
  • Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth.

Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth.

Stem cell research & therapy (2020-09-25)
Ling Leng, Jie Ma, Luye Lv, Wenjuan Wang, Dunqin Gao, Yunping Zhu, Zhihong Wu
摘要

Millions suffer from skin diseases. Functional interfollicular epidermal stem cells are needed in skin therapy or drug screening in vitro. We obtained functional interfollicular epidermal stem cells with intact stemness and cell junctions by treating them with Wnt3a. Moreover, epidermal stem cell-derived extracellular vesicles were useful in epidermal cell growth. Finally, functional epidermal 3D organoids with polarity were cultured using Wnt3a and the supernatant derived from interfollicular epidermal stem cells and fresh medium in a 1:1 ratio. These results provide novel directions for the improvement of skin organoids and their potential in clinical application.

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Sigma-Aldrich
透明质酸酶 来源于牛睾丸, Type I-S, lyophilized powder, 400-1000 units/mg solid
Sigma-Aldrich
N-乙酰基-L-半胱氨酸, BioReagent, suitable for cell culture
Sigma-Aldrich
A 83-01, ≥98% (HPLC)