技術
HPLC: suitable
測試參數
column: Ascentis Express F5, 15 cm x 4.6 mm I.D., 5 μm particles (50631-U)
mobile phase: [A] water; [B] acetonitrile; (60:40, A:B)
flow rate: 1.5 mL/min
pressure: 2727 psi (188 bar)
column temp.: 30 °C
detector: UV, 227 nm
injection: 5 μL
sample: taxols 25 μg/mL in 70:15:15, water:methanol:acetonitrile
適合性
application for HPLC
應用
pharmaceutical (small molecule)
分析報告
An efficient reverse phase HPLC method for the HPLC analysis of of Paclitaxel (Taxol) and related compounds is developed using the Ascentis® Express F5 5μm Fused Core HPLC column. Paclitaxel is a natural product with antitumor activity. TAXOL (paclitaxel) is obtained via a semi-synthetic process from Taxus baccata.
The developed method can be easily used for the routine quality control of bulk and Parenterals dosage form of Paclitaxel within a short analysis time. This method offers the advantages of higher efficies, reduced backpressure and shorter run times over a traditionally run methods over 5 μm totally porous particle columns.
The developed method can be easily used for the routine quality control of bulk and Parenterals dosage form of Paclitaxel within a short analysis time. This method offers the advantages of higher efficies, reduced backpressure and shorter run times over a traditionally run methods over 5 μm totally porous particle columns.
法律資訊
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
描述
Baccatin III, ≥95% (HPLC)
Cephalomannine, ≥97% (HPLC), solid
Paclitaxel, from Taxus yannanensis, powder
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