技術
HPLC: suitable
測試參數
column: Ascentis Express OH5, 10 cm x 3 mm I.D., 2.7 μm particles (53769-U)
mobile phase: [A] 0.1% formic acid, pH 3.5 with ammonium formate; [B] acetonitrile; (25:75, A:B)
flow rate: 0.4 mL/min
pressure: 1350 psi (93 bar)
column temp.: ambient
detector: ESI (+), scan m/z 150-300
injection: 5 μL
sample: 10 μg/mL in 90% methanol
適合性
application for HPLC
應用
chemicals and industrial polymers
分析報告
This figure shows the extracted ion currents for methionine sulfoximine-related compounds on Ascentis Express OH5. The OH5 shows excellent peak shape compared to traditional HILIC on bare silica.
法律資訊
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
描述
L-Methionine sulfoximine, suitable for cell culture
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