技術
HPLC: suitable
測試參數
column: Ascentis Express F5, 15 cm x 4.6 mm I.D., 2.7 μm particles (53591-U)
mobile phase: [A] water; [B] methanol; (50:50, A:B)
flow rate: 0.8 mL/min
pressure: 2393 psi (165 bar)
column temp.: 35 °C
detector: UV, 240 nm
injection: 5 μL
sample: 10 μg/mL of each in 90: 10, water: methanol
適合性
application for HPLC
應用
clinical
分析報告
Sample concentrations at 10 ug/mL in 90:10 water:methanol. Acetonitrile does not provide adequate selectivity on this phase. Mass spec was used for peak identification.
法律資訊
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
描述
Hydrocortisone
Prednisolone
Prednisone
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