技術
HPLC: suitable
測試參數
column: Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 μm particles (53814-U)
mobile phase: [A] 20 mM ammonium acetate, pH 4.7 with acetic acid: [B] acetonitrile; (90:10, A:B)
flow rate: 0.6 mL/min
column temp.: 40 °C
detector: UV, 214 nm
injection: 1 μL
sample: 100 - 500 μg/mL in 95:5, buffer: acetonitrile
適合性
application for HPLC
應用
food and beverages
分析報告
This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of common drink additives used in diet soda.
法律資訊
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
描述
Caffeine
Benzoic acid
Asp-Phe methyl ester
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