技術
HPLC: suitable
測試參數
column: Discovery C18, 25 cm x 4.6 mm I.D., 5 μm particles (504971)
mobile phase: [A] 1 mM potassium phosphate, monobasic; [B] acetonitrile
gradient: 5 to 45% B in 18 min; 45 to 80% B in 7 min; held at 80% B for 3 min
flow rate: 1.5 mL/min
pressure: 3191 psi (220 bar)
column temp.: 27 °C
detector: UV, 227 nm
injection: 20 μL
sample: 20 μg/mL in water: acetonitrile (86:14)
適合性
application for HPLC
應用
vitamins, nutraceuticals, and natural products
分析報告
This application demonstrates the suitability of Discovery C18 for the efficient separation of withania extract by the USP monograph.
法律資訊
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
描述
Withanoside IV
Withanoside V
Withaferin A
12-Deoxywithastramonolide
Withanolide A
Withanolide B
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