技術
HPLC: suitable
測試參數
column: Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 μm particles (53814-U)
mobile phase: [A] water with 0.1% formic acid; [B] methanol
gradient: held at 35% B for 3 min; 35 to 45% B in 10 min; held at 45% B for 2 min; 45 to 100% B in 5 min
flow rate: 0.6 mL/min
pressure: 4105 psi (283 bar)
column temp.: 35 °C
detector: UV, 254 nm
injection: 20 μL
sample: 20 mg/mL in water:ethanol (5:95); sonicate 15 minutes; filter 0.45 μm; dilute to water:ethanol (80:20)
適合性
application for HPLC
應用
vitamins, nutraceuticals, and natural products
分析報告
This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of milk thistle containing herbal supplement.
法律資訊
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
描述
Taxifolin
Silychristin
Apigenin 7-glucoside
Silydianin
Quercitrin
Silibinin
Silybin B
Isosilybin A
Isosilybin B
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分析柱
標準
產品號碼
描述
訂價
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