技術
HPLC: suitable
測試參數
column: Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)
mobile phase: [A] water; [B] acetonitrile; (60:40, A:B)
flow rate: 0.2 mL/min
pressure: 2944 psi (203 bar)
column temp.: 35 °C
detector: UV, 254 nm
injection: 2 μL
sample: 50 mg/L in 75:25, water:methanol
適合性
application for HPLC
應用
clinical
分析報告
This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of 5 steroids.
法律資訊
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
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