技術
HPLC: suitable
測試參數
column: CHIROBIOTIC R, 25 cm x 4.6 mm I.D., 5 μm particles (13024AST)
mobile phase: [A] 0.1% ammonia, pH 4.1 with formic acid; [B] methanol; (50:50, A:B)
flow rate: 1 mL/min
column temp.: ambient
detector: UV, 258 nm
injection: 10 μL
sample: 2,3-dihydroxy-3-phenyl-propionic acid isomers:
(A) racemate A (4.85 and 6.95 min)
(B) racemate B (5.33 and 6.29 min)
適合性
application for HPLC
應用
chemicals and industrial polymers
pharmaceutical (small molecule)
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